LICENSE THESIS HELD IN THE JULY 2018 SESSION

No. Student Coordinating teacher Thesis title
1 Nicoleta SĂCULŢEANU Daniela DASCĂLU, Beatrice VLAD-OROS Hydrogels based on chitosan with clinical applications
2 Maria – Doriana – Otilia CIOSICI Vasile OSTAFE, Bianca Vanesa BOROȘ Toxicity assessment of polysaccharides: the growth inhibition test on Lemna sp.
3 Elena – Roxana VÎLCEANU Vasile OSTAFE, Bianca Vanesa BOROȘ Toxicity assessment of polysaccharides: the acute immobilization assay on Daphnia sp.
4 Silvana CEAURANU Adriana ISVORAN Computational evaluation of ADME-Tox profiles of polylactic acid and polyhydroxyalkanoates

 

MASTER THESIS HELD IN THE JULY 2018 SESSION

No. Student Coordinating teacher Thesis title
1 Anca DUMITRESCU Daniela DASCĂLU, Beatrice VLAD-OROS Controlled release systems based on biopolymers
2 Ovidiu – Silviu COTREANȚI Vasile OSTAFE, Bianca Vulpe Optimization of a method of separation of mono and disaccharides by UPLC with MS-quadrupole detection
3 Monica – Elena GUȘĂ Vasile OSTAFE, Bianca Vulpe Para-nitrophenol identification methods through spectrophotometric methods and chromatographic methods, comparison between UV/VIS spectrophotometer, nanodrop and HPLC/PDA

 

 

Title

Hydrogels based on chitosan with clinical applications

Date of Award

09.07.2018

Degree Type:

Bachelor in Chemistry

Candidate:

SĂCULŢEANU Nicoleta (nicoleta.saculteanu00@e-uvt.ro;  nicoletasaculteanu@ymail.com)

, (West University of Timisoara; Faculty of Chemistry, Biology, Geography; Department of Biology – Chemistry; Specialization Chemistry

Advisor:

Lecturer PhD DASCĂLU Daniela, Lecturer PhD VLAD-OROS Beatrice (West University of Timisoara, Department of Biology – Chemistry, Pestalozzi 16, Timisoara 300115, Romania; Advanced Environmental Research Laboratories, Oituz 4, Timisoara 300086, Romania)

Abstract:

In this study we evaluated the influence of how xerogels were produced on the controlled release of a drug. A controlled release system should release the drug into the target organ at a rate appropriate to the specific treatment. Generally, these systems distribute the active substance uniformly into the gastrointestinal tract in order to reduce mucosal irritation. Chitosan is a natural polysaccharide that is commonly used to obtain controlled release systems due to its biocompatibility and lack of toxicity. As a model drug in this study, a fluoroquinolone was used, namely ciprofloxacin, a compound that has good bactericidal activity against uropathogenic bacterial agents and that is indicated in the treatment of urinary tract infections, intra-abdominal infections, infectious diarrhea, typhoid fever, gonorrhea, etc. Controlled release systems with ciprofloxacin allow exposure to a drug comparable to that obtained with conventional, twice-daily, immediate-release administration. Xerogels obtained have good reproductibility. The behavior of xerogels in acidic (pH = 1) and slightly alkaline (pH = 7.4) medium was studied and the influence of how they were obtained on the controlled release of a drug was evaluated. The kinetics of controlled drug release correspond to the Higuchi model.

Recommended Citation:

SĂCULŢEANU Nicoleta, „Hydrogels based on chitosan with clinical applications”, Bachelor Thesis, West University of Timișoara, 2018

 

Title

Toxicity assessment of polysaccharides: the growth inhibition test on Lemna sp.

Date of Award

09.07.2018

Degree Type:

Bachelor in Biology

Candidate:

CIOSICI, Maria-Doriana-Otilia (ciosicidoriana8@gmail.com) (West University of Timisoara; Faculty of Chemistry, Biology, Geography; Department of Biology – Chemistry; Specialization Biology)

Advisor:

Prof. Dr. Vasile OSTAFE

Drd. Bianca-Vanesa BOROS

(West University of Timisoara, Faculty of Chemistry, Biology, Geography, Department of Biology – Chemistry, Pestalozzi 16, Timisoara 300115, Romania; Advanced Environmental Research Laboratories, Oituz 4, Timisoara 300086, Romania)

Abstract:

A bibliographic study on natural and semisynthetic polysaccharides has been made, focusing on chitin and chitosan, cellulose and carboxymethyl cellulose (CMC), alginate and starch. Ecotoxicity as well as widely used test methods and guided by international standards have been described. Various types of organisms used in such tests have been described. Also described were the organisms used for the growth inhibition assay studied in this thesis.

Multiple experiments have been conducted to optimize / adapt the test and the duckweed culture to the conditions that can be provided in the laboratory. Ecotoxicity tests have also been carried out to determine the effect of chitosan, alginate, carboxymethyl cellulose and starch polysaccharides on Lemna sp. The results calculated were the number of fronds and different indexes such as growth inhibition percentage or EC50.

The results revealed that of all four polysaccharides, chitosan had the most toxic effect, followed by alginate. Starch had the lowest effect, and for CMC the necessary calculations couldn’t be made due to the concentrations selected for testing.

Recommended Citation:

CIOSICI, Maria-Doriana-Otilia, „Toxicity assessment of polysaccharides: the growth inhibition test on Lemna sp.”, Bachelor Thesis, West University of Timișoara, 2018

 

Title

Toxicity assessment of polysaccharides: the acute immobilization assay on Daphnia sp.

Date of Award

09.07.2018

Degree Type:

Bachelor in Biology

Candidate:

VÎLCEANU, Elena-Roxana (roxaele@yahoo.com) (West University of Timisoara; Faculty of Chemistry, Biology, Geography; Department of Biology – Chemistry; Specialization Biology)

Advisor:

Prof. Dr. Vasile OSTAFE

Drd. Bianca-Vanesa BOROS

(West University of Timisoara, Faculty of Chemistry, Biology, Geography, Department of Biology – Chemistry, Pestalozzi 16, Timisoara 300115, Romania; Advanced Environmental Research Laboratories, Oituz 4, Timisoara 300086, Romania)

Abstract:

                A literature study on natural and semi-synthetic polysaccharides was carried out by describing the classification, properties, applications and some examples. The polysaccharides of interest in this paper are chitosan, alginate, carboxymethyl cellulose and starch. The ecotoxicity tests for aquatic animals have also been described, more precisely for daphnia. The testing organisms were also described in turn. Several experiments have been carried out: some to optimize others for ecotoxicity. The four polysaccharides of different concentrations were tested at 24 and 48 hours. Based on the results obtained, percent mortality and EC50 for all polysaccharides were calculated if possible.

                The results obtained showed that of all four polysaccharides, the alginate had the most toxic effect, followed by starch. Carboxymethyl cellulose had the lowest effect.

Recommended Citation:

VÎLCEANU, Elena-Roxana, „Toxicity assessment of polysaccharides: the acute immobilization assay on Daphnia sp.”, Bachelor Thesis, West University of Timișoara, 2018

 

Title

Computational evaluation of ADME-Tox profiles of polylactic acid and polyhydroxyalkanoates

Date of Award

09.07.2018

Degree Type:

Bachelor in Biochemistry

Candidate:

CEAURANU Silvana (ceauranu_silvana@yahoo.com), West University of Timisoara; Faculty of Chemistry, Biology, Geography; Department of Biology – Chemistry; Specialization Biochemistry.

Advisor:

Prof. Dr. Adriana ISVORAN (West University of Timisoara, Department of Biology – Chemistry, Pestalozzi 16, Timisoara 300115, Romania; Advanced Environmental Research Laboratories, Oituz 4, Timisoara 300086, Romania)

Abstract:

This paper contains a study on the pharmacological properties of lactic acid oligomers L (PLA) and polyhydroxyalkanoates, namely polyhydroxybutyrates (PHB), polyhydroxy valerates (PHV) and PHBV copolymers. The computational programs SwissADME and admetSAR were used. The predictions made with the two programs are in good agreement with each other and, in some cases with literature data, when they exist. The ability of these compounds to penetrate the skin is reduced and decreases with increasing molecular weight, the highest penetration capacity through the skin has the PHV oligomers. Investigated oligomers reveal high gastrointestinal absorption if the number of monomers is small (up to 4 monomers). The investigated oligomers are considered non-P-gp non-inhibitors and possible substrates for this, which illustrates that their systemic exposure may be reduced. The vast majority of the investigated oligomers do not have the ability to inhibit human cytochromes and are not substrates for them, have no mutagenic, carcinogenic potential, and do not reflect cardiotoxicity.All these results show that PLA and PHA oligomers do not have toxic potential for the human body, reveal low toxicity to the environment and high biodegradability. This result is particularly important for those who are professionally exposed to these substances and for the fact that their waste does not produce toxic effects on the environment.
CEAURANU Silvana, „Computational evaluation of ADME-Tox profiles of polylactic acid and polyhydroxyalkanoates”, Bachelor Thesis, West University of Timișoara, 2018

 

Title

Controlled release systems based on biopolymers

Date of Award

13.07.2018

Degree Type:

Master in Chemistry

Candidate:

DUMITRESCU Anca (ancadumitrescu_umft@yahoo.com), (West University of Timisoara; Faculty of Chemistry, Biology, Geography; Department of Biology – Chemistry; Specialization Clinical and laboratory chemistry

Advisor:

Lecturer PhD DASCĂLU Daniela, Lecturer PhD VLAD-OROS Beatrice (West University of Timisoara, Department of Biology – Chemistry, Pestalozzi 16, Timisoara 300115, Romania; Advanced Environmental Research Laboratories, Oituz 4, Timisoara 300086, Romania)

Abstract:

The major advantages of controlled-release biopolymer matrices for active substance delivery are: transport of the active substance directly to the target organ, its sustained distribution and the fact that the encapsulated therapeutic agent is released over long periods of time and therefore it eliminated the need for multiple administrations. The purpose of the experimental part was to obtain an encapsulation formulation of ciprofloxacin in chitosan and chitosan and alginate microspheres, respectively, for its prolonged release. Two types of polymeric hydrogels were prepared by ionic crosslinking with sodium tripolyphosphate. The first type of hydrogel consists only of chitosan, and the second type of hydrogel consists of two layers: the inner layer made of chitosan and the outer layer made of alginate. Both the starting materials and the microspheres were characterized using FT-IR spectroscopy. The dimensions of the microspheres, entrapping efficiency and water absorption capacity were determined. The kinetics of ciprofloxacin release was studied using four models (order 0, order 1, Higuci and Hixson-Crowell). The average size of the microspheres was 2.62 ± 0.11 mm for those with chitosan, 2.98 ± 0.14 mm for those consisting of both chitosan and alginate. After drying, their size was reduced by about half. The swelling capacity of the microspheres depends on the type of microspheres, on the humidity, namely if the determination was performed on wet or dry microspheres and on the pH of the medium in which the determination was made. In this study we evaluated a variable that can alter the release of the active substance from a chitosan-based matrix. The coating of chitosan microspheres with an alginate layer determines a slower and lower release of the active substance, but the alginate layer protects the chitosan microspheres from disintegration in 0.1N HCl solution pH 1. At pH 7.4, chitosan is insoluble, so these matrices are more suitable for releasing the active substance in the intestine.

Recommended Citation:

DUMITRESCU Anca, „Release systems based on biopolymers”, Master Thesis, West University of Timișoara, 2018

 

Title

Optimization of a method of separation of mono and disaccharides by UPLC with MS-quadrupole detection

Date of Award

13.07.2018

Degree Type:

Master in Chemistry

Candidate:

Ovidiu-Silviu COTREANȚI (cotreantisilviu@yahoo.com), (West University of Timisoara; Faculty of Chemistry, Biology, Geography; Department of Biology – Chemistry; Specialization Clinical and laboratory chemistry

Advisor:

Prof. Dr. Vasile Ostafe, PhD Bianca Vulpe (West University of Timisoara, Department of Biology – Chemistry, Pestalozzi 16, Timisoara 300115, Romania; Advanced Environmental Research Laboratories, Oituz 4, Timisoara 300086, Romania)

Abstract:

The work was done in two major parts. The first part contains 5 chapters presenting the generalities of ultra-high performance liquid chromatography, the necessary equipment for the separation analysis and the necessary equipment for the detection of the separated analytes. In the second part, a study was conducted on optimal parameters for the separation of the two monosaccharides, namely fructose and glucose, and sucrose, being a disaccharide. As a result, we can see that the separation was successful. The purpose of this paper was to optimize a method of separating saccharide compounds in a much easier and faster way than conventional methods

Recommended Citation:

COTREANȚI, Ovidiu-Silviu, „ Optimization of a method of separation of mono and disaccharides by UPLC with MS-quadrupole detection”, Master Thesis, West University of Timișoara, 2018

 

Title

Para-nitrophenol identification methods through spectrophotometric methods and chromatographic methods, comparison between UV/VIS spectrophotometer, nanodrop and HPLC/PDA

Date of Award

13.07.2018

Degree Type:

Master in Chemistry

Candidate:

Monica Elena GUȘĂ (monica.elena14@gmail.com), (West University of Timisoara; Faculty of Chemistry, Biology, Geography; Department of Biology – Chemistry; Specialization Clinical and laboratory chemistry

Advisor:

Prof. Dr. Vasile Ostafe, PhD Bianca Vulpe (West University of Timisoara, Department of Biology – Chemistry, Pestalozzi 16, Timisoara 300115, Romania; Advanced Environmental Research Laboratories, Oituz 4, Timisoara 300086, Romania)

Abstract:

Ultra High Performance Liquid Chromatography is a technique used to identify and quantitate certain compounds in a mixture without separating them before analysis.

The purpose of this paper is to prove that other techniques, such as UV-VIS Spectrophotometer, Nanodrop or Spectrophotometry for microplates (ELISA), are not as precise as chromatography. To demonstrate this, calibration strains were drawn using a chromofor compound – para-nitrophenol. The studied parameters were the coefficient of regression and the aspect of the straight line

Recommended Citation:

GUSĂ, Monica-Elena, „Para-nitrophenol identification methods through spectrophotometric methods and chromatographic methods, comparison between UV/VIS spectrophotometer, nanodrop and HPLC/PDA”, Master Thesis, West University of Timișoara, 2018